3D Ultrastructural Imaging dedicated to host pathogen studies

P. BOMME*, M. MAJROUH, E. PENARD, D TAHIR, V CHOUMET, Jost ENINGA, G DUMENIL, A.MALLET 

Institut Pasteur, 28 Rue du Dr Roux, 75015 Paris

Microscopy and imaging in general are crucial points for scientist to understand a specific biological event as entry of a pathogen into cells or tissue and how vectors transmit diseases.

Electron microscopy (EM) has always been a method of choice to reach ultrastructural details [1]
More recently 3D EM technics can enhance and sometimes revolutionize how we view cells and biological processes [2].
In the last years, 3D imaging modalities became one of the main challenges for electron imaging community.

In our facility UBI, we proposed a large choice of 3D methods allowing to visualize proteins, virus, cells or tissues. We’ll discuss about 3D methods applied to biological samples and how 3D reconstruction allows better understanding of pathogen associated processes.

References:
[1] Santos JC et al. 2015
[2] Mallet et al. 2022